8V1J

Structure of an allelic variant of Puccinia graminis f. sp. tritici (Pgt) effector AvrSr27 (AvrSr27-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.26
Spacegroup name	P 1 21 1
Unit cell lengths	43.650, 34.300, 70.740
Unit cell angles	90.00, 92.90, 90.00

Refinement procedure

Resolution	43.594 - 2.416
R-factor	0.212545654587
Rwork	0.208
R-free	0.25312
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.520
Data scaling software	Aimless (7.0.78)
Phasing software	CRANK2 (7.0.78)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.594	2.510
High resolution limit [Å]	2.416	2.420
Number of reflections	8197	811
<I/σ(I)>	11	3.9
Completeness [%]	99.2	95.5
Redundancy	15.7
CC(1/2)	0.997	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium acetate, 0.1 M Tris pH 7.5, 30% w/v PEG4000, with 10% w/v glycerol