8V1H
Crystal structure of human pre-mascRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-07-29 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 44.986, 40.651, 123.393 |
| Unit cell angles | 90.00, 96.99, 90.00 |
Refinement procedure
| Resolution | 40.830 - 2.890 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.24590 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.062 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.830 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 5028 | 491 |
| <I/σ(I)> | 9.26 | |
| Completeness [%] | 94.5 | |
| Redundancy | 11.6 | |
| CC(1/2) | 0.998 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M sodium chloride, 0.1 M lithium chloride, 0.01 M magnesium chloride hexahydrate, 0.05 M MES (pH 6.5) and 35% MPD |
