8V14

Structure of NRAP-1 and its role in NMDAR signaling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	41.358, 75.921, 152.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.120 - 1.900
R-factor	0.1954
Rwork	0.194
R-free	0.22890
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.828
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	SOLVE
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.120	38.100	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.111	0.047	5.366
Rmeas	0.112	0.047	5.420
Rpim	0.015	0.007	0.759
Total number of observations		19511	122697
Number of reflections	38804	431	2443
<I/σ(I)>	27.4	81.2	1.2
Completeness [%]	100.0	99.2	100
Redundancy	54.8	45.3	50.2
CC(1/2)	1.000	0.999	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.35	277	17 mg/mL purified NRAP-1 in 150 mM sodium chloride, 15 mM HEPES, 1 mM calcium chloride, pH 7.35, 1:1 with crystallization solution (26% PEG8000, 100 mM CHES, pH 9.8)