8V0J

Structure of the complex between Human LIAS and H-protein in the presence of s-adenosyl-l-methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-11-13
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	49.260, 171.377, 196.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.280 - 2.580
R-factor	0.1991
Rwork	0.198
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.519
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.620
High resolution limit [Å]	2.580	2.580
Number of reflections	53713	2928
<I/σ(I)>	8
Completeness [%]	91.7
Redundancy	7.3
CC(1/2)	0.977	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM potassium phosphate, 200 mM potassium chloride, 35% (w/v) PEG 3350, 1 mM s-adenosyl-l-methionine