8V0E
ANK repeat of MIB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.997, 137.470, 96.248 |
| Unit cell angles | 90.00, 108.14, 90.00 |
Refinement procedure
| Resolution | 47.640 - 2.390 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.943 |
| Data reduction software | HKL-2000 (1.20.1_4487) |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.740 | 2.440 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rpim | 0.062 | 0.568 |
| Number of reflections | 37769 | 1868 |
| <I/σ(I)> | 14.29 | 1.12 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 4.6 | 4.3 |
| CC(1/2) | 0.995 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1M Ammonium tartrate dibasic, 0.1M Sodium acetate trihydrate pH 4.6 |
