8UZF
Crystal structure of chimeric RaTG13 RBD complexed with chimeric mouse ACE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.556, 119.004, 109.075 |
| Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
| Resolution | 108.490 - 3.283 |
| R-factor | 0.2259 |
| Rwork | 0.223 |
| R-free | 0.27950 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond angle | 10.899 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.490 | 3.717 |
| High resolution limit [Å] | 3.283 | 3.283 |
| Rmerge | 0.150 | 0.905 |
| Rmeas | 0.175 | 1.062 |
| Rpim | 0.089 | 0.550 |
| Total number of observations | 2855 | |
| Number of reflections | 15465 | 773 |
| <I/σ(I)> | 5 | 1.4 |
| Completeness [%] | 50.5 | |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM Tris, pH 8.5, 18-22% PEG6000, 100 mM sodium chloride |
