8UZE
Crystal structure of chimeric bat coronavirus BANAL-20-236 RBD complexed with chimeric mouse ACE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.142, 118.211, 107.860 |
| Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
| Resolution | 42.430 - 3.030 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.26130 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond angle | 10.920 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.266 | 3.457 |
| High resolution limit [Å] | 3.030 | 3.046 |
| Rmerge | 0.074 | 0.695 |
| Rmeas | 0.085 | 0.788 |
| Rpim | 0.042 | 0.368 |
| Total number of observations | 4814 | |
| Number of reflections | 21150 | 1058 |
| <I/σ(I)> | 7 | 1.7 |
| Completeness [%] | 54.7 | |
| Redundancy | 4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM Tris, pH 8.5, 18-22% PEG6000, 100 mM sodium chloride |
