8UX6
Structure of Fab201 with a T. parva sporozoite neutralizing B cell epitope of p67
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 1 |
Unit cell lengths | 39.010, 75.560, 90.591 |
Unit cell angles | 69.26, 77.60, 76.08 |
Refinement procedure
Resolution | 19.520 - 2.000 |
R-factor | 0.2058 |
Rwork | 0.204 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.943 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.850 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.068 | 0.241 |
Rmeas | 0.091 | 0.321 |
Rpim | 0.059 | 0.210 |
Number of reflections | 54860 | 4175 |
<I/σ(I)> | 7.8 | 3.4 |
Completeness [%] | 87.3 | 88.5 |
Redundancy | 2.2 | 2.2 |
CC(1/2) | 0.987 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 25% PEG6000, 0.1 M HEPES pH 7.0 and 0.2 M Calcium Chloride |