8UX6
Structure of Fab201 with a T. parva sporozoite neutralizing B cell epitope of p67
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.010, 75.560, 90.591 |
| Unit cell angles | 69.26, 77.60, 76.08 |
Refinement procedure
| Resolution | 19.520 - 2.000 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.943 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.850 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.241 |
| Rmeas | 0.091 | 0.321 |
| Rpim | 0.059 | 0.210 |
| Number of reflections | 54860 | 4175 |
| <I/σ(I)> | 7.8 | 3.4 |
| Completeness [%] | 87.3 | 88.5 |
| Redundancy | 2.2 | 2.2 |
| CC(1/2) | 0.987 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 25% PEG6000, 0.1 M HEPES pH 7.0 and 0.2 M Calcium Chloride |
