8UVK
CosR DNA bound form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.620, 80.101, 68.202 |
| Unit cell angles | 90.00, 105.60, 90.00 |
Refinement procedure
| Resolution | 65.690 - 2.210 |
| R-factor | 0.2542 |
| Rwork | 0.253 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.114 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.690 | 2.289 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 33515 | 3406 |
| <I/σ(I)> | 9.85 | |
| Completeness [%] | 96.4 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.996 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.03 M Magnesium chloride, 0.03 M Calcium chloride, 0.1 M MES ph6.5, 40% v/v Ethylene glycol, 20% w/v PEG 8000 |
