8UUT
Structure of Serratia entomophila antifeeding prophage Fibre foot domain (Afp13)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-11-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 130.559, 130.559, 76.409 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.450 - 1.830 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.929 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.450 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rpim | 0.073 | 0.622 |
| Number of reflections | 64474 | 3940 |
| <I/σ(I)> | 9.8 | 1.8 |
| Completeness [%] | 97.4 | 96.3 |
| Redundancy | 43 | 42.5 |
| CC(1/2) | 0.996 | 0.742 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.6 M Tri sodium citrate |
