8UUS
Structure of Serratia proteamaculans antifeeding prophage Fibre foot domain (AfpX13)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-03-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.953725 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 59.113, 68.241, 111.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.620 - 1.470 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.912 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 1.490 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rpim | 0.061 | 1.112 |
| Number of reflections | 77498 | 3684 |
| <I/σ(I)> | 10 | 0.8 |
| Completeness [%] | 99.9 | 97.1 |
| Redundancy | 13.6 | 13.4 |
| CC(1/2) | 0.999 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 16% PEG 3350, 0.15 M Ammonium citrate |
