8UUR

Structure of Serratia proteamaculans antifeeding prophage mini-fibre (AfpX13)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.953725
Spacegroup name	C 1 2 1
Unit cell lengths	118.556, 68.409, 132.757
Unit cell angles	90.00, 93.32, 90.00

Refinement procedure

Resolution	45.470 - 2.500
R-factor	0.2197
Rwork	0.217
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.035
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.470	2.600
High resolution limit [Å]	2.500	2.500
Rpim	0.124	0.668
Number of reflections	36902	4143
<I/σ(I)>	5.8	1.5
Completeness [%]	99.7	99.9
Redundancy	4.2	3.9
CC(1/2)	0.984	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	8% PEG 3350, 0.1 M HEPES pH 7.5, 20 mM CdCl2