8US4
C2221 Crystal structure of TamA (Barrel only) from Pseudomonas aeruginosa at 3.15 Ang
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-10-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97910 Ang |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.975, 149.876, 53.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.987 - 3.150 |
| Rwork | 0.300 |
| R-free | 0.35000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS (3.3.20) |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.988 | 48.980 | 3.390 |
| High resolution limit [Å] | 3.150 | 5.390 | 3.150 |
| Rmerge | 0.065 | 0.580 | |
| Rmeas | 0.244 | ||
| Rpim | 0.121 | 0.050 | 0.383 |
| Number of reflections | 12152 | 6121 | 5704 |
| <I/σ(I)> | 7.5 | 4.5 | 1.2 |
| Completeness [%] | 97.8 | 93.4 | 97.9 |
| Redundancy | 3.9 | 3.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 12% peg 4K, 50 mM MgCl2, 70 mM Hepes pH 7.2, 4% isopropanol, 10% glycerol |
