8US3

C2 Crystal structure of TamA from Pseudomonas aeruginosa at 3.1 Ang

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	CCD
Collection date	2013-10-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97910 Ang
Spacegroup name	C 1 2 1
Unit cell lengths	140.400, 63.880, 81.360
Unit cell angles	90.00, 99.40, 90.00

Refinement procedure

Resolution	48.600 - 3.100
Rwork	0.250
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.600	48.600	3.900
High resolution limit [Å]	3.100	6.900	3.100
Number of reflections	13130	805	6674
<I/σ(I)>	11.2
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.900	0.990	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	295	12% peg 4K, 50 mM MgCl2, 70 mM Hepes pH 7.2, 4% isopropanol, 10% glycerol