8US2
P22121 Crystal structure of TamA from Pseudomonas aeruginosa at 3.95 Ang
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-10-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97910 Ang |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 73.050, 155.502, 157.021 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.255 - 3.955 |
R-factor | 0.306 |
Rwork | 0.302 |
R-free | 0.38130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.399 |
Data reduction software | XDS (3.3.20) |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.063 | 44.063 | 4.170 |
High resolution limit [Å] | 3.955 | 12.510 | 3.955 |
Rmerge | 0.031 | 0.871 | |
Rmeas | 0.309 | ||
Rpim | 0.155 | 0.022 | 0.590 |
Total number of observations | 62873 | ||
Number of reflections | 16231 | 870 | 3507 |
<I/σ(I)> | 7.2 | 13.5 | 0.8 |
Completeness [%] | 99.7 | 94.9 | 99.7 |
Redundancy | 3.9 | 3.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 12% peg 4K, 50 mM MgCl2, 70 mM Hepes pH 7.2, 4% isopropanol, 10% glycerol |