8US1

P21 Crystal structure of TamA from Pseudomonas aeruginosa at 2.6 Ang

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	CCD
Collection date	2013-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97910 Ang
Spacegroup name	P 1 21 1
Unit cell lengths	78.710, 63.843, 84.010
Unit cell angles	90.00, 108.55, 90.00

Refinement procedure

Resolution	40.900 - 2.650
Rwork	0.200
R-free	0.26000
Structure solution method	SAD
Data reduction software	autoXDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.650	7.190	2.650
Rmerge	0.115	0.046
Number of reflections	41274	1537	2793
<I/σ(I)>	12.6
Completeness [%]	93.2	99.4	83.4
Redundancy	4.1	5.8	2.8
CC(1/2)			0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	12% peg 4K, 50 mM MgCl2, 70 mM Hepes pH 7.2, 4% isopropanol, 10% glycerol