8US0

Human antibody S8V1-157 in complex with the A/American black duck/New Brunswick/00464/2010(H4N6) HA head domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	127.920, 241.540, 144.130
Unit cell angles	90.00, 101.62, 90.00

Refinement procedure

Resolution	49.330 - 3.700
R-factor	0.2481
Rwork	0.246
R-free	0.29260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.330	3.836
High resolution limit [Å]	3.700	3.704
Number of reflections	89908	8941
<I/σ(I)>	7	1.2
Completeness [%]	98.8	98.49
Redundancy	2	2
CC(1/2)	0.994	0.447

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.1 M Lithium sulfate; 0.1 M Sodium chloride 0.1 M MES pH 6.5 30% (v/v) PEG 400