8UQT

Crystal structure of the Tree Shrew p53 tetramerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	C 2 2 2
Unit cell lengths	62.871, 65.806, 32.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.460 - 1.160
R-factor	0.1732
Rwork	0.172
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.154
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.460	1.201
High resolution limit [Å]	1.160	1.160
Number of reflections	21880	21822
<I/σ(I)>	28.35
Completeness [%]	91.7
Redundancy	9.9
CC(1/2)	1.000	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.6 M Lithium sulfate monohydrate, 2% PEG 8000, 10% glycerol