8UQH

X-ray crystal structure of PRMT4 bound to compound YD-1130

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-08
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9774
Spacegroup name	P 21 21 2
Unit cell lengths	74.726, 98.728, 206.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.710 - 1.870
R-factor	0.215
Rwork	0.214
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.598
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.810	1.900
High resolution limit [Å]	1.870	1.870
Rmerge	0.191
Rmeas	0.208
Rpim	0.080
Number of reflections	124972	5739
<I/σ(I)>	4.9
Completeness [%]	98.8	92.25
Redundancy	6.6	5.73
CC(1/2)	0.989	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	7.5% (w/v) PEG 20,000, 0.1M Tris, 0.08M sodium formate, pH 7.5