8UQ9

Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 4-residue linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 31
Unit cell lengths	108.135, 108.135, 114.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.180 - 2.300
R-factor	0.184
Rwork	0.183
R-free	0.20560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gb0 5egg
RMSD bond length	0.002
RMSD bond angle	0.524
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.092	0.639
Rmeas	0.098	0.685
Rpim	0.035	0.244
Number of reflections	66052	6543
<I/σ(I)>	21	2.59
Completeness [%]	99.8	99.54
Redundancy	7.8	7.8
CC(1/2)	0.999	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	Crystals were obtained by mixing 2 microliters of the protein fusion (8.8 mg/mL) in 10 mM HEPES, pH 7.5, 600 mM NaCl, 1 mM TCEP and 2 microliters of the reservoir solution containing 0.1 M HEPES, pH 7.5, 2.4 M NaCl