8UOM

LSD1-CoREST with N-formyl-FAD in complex with H3dimeK4 histone tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.999998
Spacegroup name	I 2 2 2
Unit cell lengths	117.673, 177.827, 234.528
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.070 - 3.200
R-factor	0.2186
Rwork	0.217
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.495
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.070	3.330
High resolution limit [Å]	3.200	3.200
Rmerge	0.195	2.176
Rmeas	0.211	2.360
Rpim	0.081	0.905
Total number of observations	277958	30354
Number of reflections	40894	4558
<I/σ(I)>	7.1	0.9
Completeness [%]	99.9
Redundancy	6.8	6.7
CC(1/2)	0.996	0.489

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.2 Na/K Tartrate, 100 mM ADA pH 6.5