8UMO
Murine CD94-NKG2A receptor in complex with Qa-1b presenting AMAPRTLLL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.170, 65.091, 198.574 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.460 - 2.600 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.460 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 0.820 |
| Number of reflections | 21632 | 12725 |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 23% PEG3350 and 0.2M NaBr |
