8ULZ
LSD1-CoREST in complex with T18 and SNAG peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.967697 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.371, 177.771, 234.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.490 - 3.320 |
| R-factor | 0.2006 |
| Rwork | 0.199 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.478 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.427 | 3.112 |
| High resolution limit [Å] | 2.808 | 2.808 |
| Rmerge | 0.143 | 1.932 |
| Rmeas | 0.149 | 2.004 |
| Rpim | 0.040 | 0.530 |
| Total number of observations | 562400 | 28662 |
| Number of reflections | 41073 | 2055 |
| <I/σ(I)> | 13.3 | 1.5 |
| Completeness [%] | 67.0 | |
| Redundancy | 13.7 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.2 Sodium Potassium Tartrate, 100 mM ADA pH 6.5 |
