8ULH

Fab of RSV neutralizing antibody 1G12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	63.456, 82.563, 112.234
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.080 - 1.690
R-factor	0.1951
Rwork	0.194
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.205
Data reduction software	HKL-3000 (1.20.1_4487)
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.080	1.753
High resolution limit [Å]	1.690	1.692
Number of reflections	66305	4543
<I/σ(I)>	15.67
Completeness [%]	95.1
Redundancy	6.3
CC(1/2)	0.998	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	10 mM Hepes pH 7.5, 150 mM NaCl, 5% glycerol