8ULF
Crystal structure of Plasmodium vivax CelTOS in complex with antibody 7g7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 182.890, 78.620, 47.320 |
| Unit cell angles | 90.03, 95.37, 89.88 |
Refinement procedure
| Resolution | 48.000 - 3.200 |
| R-factor | 0.2783 |
| Rwork | 0.275 |
| R-free | 0.29540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.639 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 39227 | 4047 |
| <I/σ(I)> | 10.31 | |
| Completeness [%] | 90.6 | |
| Redundancy | 2.11 | |
| CC(1/2) | 0.990 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium acetate, pH 4.5, and 15% PEG 4000 |
