8ULB
LSD1-CoREST in complex with T17, long soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.151, 179.546, 236.481 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 106.410 - 3.410 |
| R-factor | 0.1931 |
| Rwork | 0.192 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.661 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.410 | 3.570 |
| High resolution limit [Å] | 3.410 | 3.410 |
| Rmerge | 0.244 | 3.071 |
| Rmeas | 0.253 | 3.192 |
| Rpim | 0.068 | 0.860 |
| Total number of observations | 470790 | 56109 |
| Number of reflections | 34601 | 4207 |
| <I/σ(I)> | 10.8 | 0.9 |
| Completeness [%] | 98.7 | |
| Redundancy | 13.6 | 13.3 |
| CC(1/2) | 0.997 | 0.389 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.2 M Sodium Potassium Tartrate, 100 mM ADA pH 6.5 |
