8ULA
Lmo2839 ABC transporter substrate binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 95.881, 95.881, 78.702 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.120 - 2.000 |
| R-factor | 0.1894 |
| Rwork | 0.188 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.523 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.120 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.350 | 0.566 |
| Rpim | 0.235 | 0.390 |
| Number of reflections | 58116 | 2649 |
| <I/σ(I)> | 2.5 | 1.9 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 3.5M NH4SO4, 0.1M MES sodium salt pH 6.5, and 1% (w/v) MPD |
