8UL8

LSD1-CoREST in complex with T15, short soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.965459
Spacegroup name	I 2 2 2
Unit cell lengths	118.527, 178.972, 235.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.540 - 2.820
R-factor	0.2251
Rwork	0.224
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.696
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.540	2.900
High resolution limit [Å]	2.820	2.820
Rmerge	0.106	1.489
Rmeas	0.129	1.804
Rpim	0.072	0.998
Total number of observations	170896	14116
Number of reflections	58656	4666
<I/σ(I)>	7.8	0.8
Completeness [%]	97.5
Redundancy	2.9	3
CC(1/2)	0.978	0.303

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.2 Na/K Tartrate, 100 mM ADA pH 6.5