8UL6
LSD1-CoREST in complex with T16, long soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-02 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.965459 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 118.316, 178.421, 234.067 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.380 - 2.740 |
| R-factor | 0.2177 |
| Rwork | 0.217 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.387 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.380 | 2.810 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.116 | 2.520 |
| Rmeas | 0.131 | 2.848 |
| Rpim | 0.058 | 1.279 |
| Total number of observations | 283696 | 17646 |
| Number of reflections | 63352 | 4174 |
| <I/σ(I)> | 8.8 | 1 |
| Completeness [%] | 97.7 | |
| Redundancy | 4.5 | 4.2 |
| CC(1/2) | 0.997 | 0.429 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.2 Sodium Potassium Tartrate, 100 mM ADA pH 6.5 |
