8UJL

Crystal structure of human CTDNEP1-NEP1R1 protein phosphatase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	C 2 2 21
Unit cell lengths	58.151, 98.849, 103.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.080 - 1.910
R-factor	0.1917
Rwork	0.191
R-free	0.21190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.563
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.080	1.976
High resolution limit [Å]	1.908	1.908
Rmerge	0.051	0.795
Rmeas	0.072	1.125
Rpim	0.051	0.795
Number of reflections	23447	2260
<I/σ(I)>	7.92	0.83
Completeness [%]	99.7	98.01
Redundancy	2	2
CC(1/2)	0.998	0.412

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	6% PEG 3350, 0.2 M lithium citrate, 0.4% CHAPS