8UIO

anti-Phosphohistidine Fab hSC44.ck

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03317
Spacegroup name	P 21 21 2
Unit cell lengths	93.818, 138.848, 73.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.910 - 1.750
R-factor	0.2118
Rwork	0.210
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.830
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.910	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.124	1.170
Rmeas	0.133	1.290
Rpim	0.049	0.530
Number of reflections	95210	4089
<I/σ(I)>	16.3	1.36
Completeness [%]	98.1	85.3
Redundancy	7.2	5.5
CC(1/2)	1.000	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Lithium citrate, 20% PEG 3350