8UIG
anti-Phosphohistidine Fab hSC44.ck.20 with 3pHis peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-25 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.111, 72.605, 70.325 |
| Unit cell angles | 90.00, 98.21, 90.00 |
Refinement procedure
| Resolution | 34.820 - 1.900 |
| R-factor | 0.1742 |
| Rwork | 0.172 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.769 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.820 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.162 | 1.200 |
| Rmeas | 0.183 | 1.410 |
| Rpim | 0.082 | 0.719 |
| Number of reflections | 33299 | 1333 |
| <I/σ(I)> | 12 | 1.8 |
| Completeness [%] | 96.1 | 77.1 |
| Redundancy | 4.5 | 3.6 |
| CC(1/2) | 0.983 | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M tri-potassium citrate, 20% PEG 3350 |
