8UHT

anti-Phosphohistidine Fab hSC44.ck.20.elbow bound to Glu H1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-02
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	P 21 21 21
Unit cell lengths	49.745, 73.275, 148.455
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.170 - 2.450
R-factor	0.2465
Rwork	0.244
R-free	0.28950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.712
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.170	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.063	0.854
Rmeas	0.069	0.941
Rpim	0.027	0.387
Number of reflections	19822	839
<I/σ(I)>	29.4	1.98
Completeness [%]	96.3	84.2
Redundancy	6.5	5.7
CC(1/2)	0.998	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tris, pH 8.5, 8% PEG8000