8UHJ
anti-Phosphohistidine Fab hSC44.ck.20.N32F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-24 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.471, 73.253, 96.106 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.050 - 1.940 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.657 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.050 | 1.980 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.180 | 2.380 |
| Rmeas | 0.193 | 2.540 |
| Rpim | 0.067 | 0.884 |
| Number of reflections | 36687 | 1806 |
| <I/σ(I)> | 12.6 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.8 | 7.6 |
| CC(1/2) | 0.993 | 0.374 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris, pH 8.5, 0.2M lithium sulfate, 40% PEG400 |
