8UHH

anti-Phosphohistidine Fab hSC44.ck.elbow.20.N32F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-24
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	P 21 21 21
Unit cell lengths	48.574, 72.072, 152.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.270 - 1.840
R-factor	0.2055
Rwork	0.204
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.861
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.270	1.880
High resolution limit [Å]	1.840	1.840
Rmerge	0.100	1.780
Rmeas	0.108	2.000
Rpim	0.042	0.904
Number of reflections	46607	2191
<I/σ(I)>	21	0.86
Completeness [%]	99.2	94.4
Redundancy	6.3	4.5
CC(1/2)	0.996	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES, pH 7.5, 20% PEG4000, 10% 2-propanol