8UGC
FD15: Flat repeat helix-turn-helix-turn protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-26 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.666, 122.784, 89.995 |
| Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
| Resolution | 89.200 - 4.000 |
| R-factor | 0.4659 |
| Rwork | 0.465 |
| R-free | 0.49170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.326 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4761) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.200 | 4.210 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.450 | 1.622 |
| Rmeas | 2.640 | |
| Rpim | 0.184 | 0.648 |
| Number of reflections | 21962 | 1648 |
| <I/σ(I)> | 5.15 | |
| Completeness [%] | 93.2 | 95.97 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 0.995 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-Tris, pH 6.5, 25% w/v PEG3350 |
