8UFN

Crystal Structure of neuronal HAstV VA1 capsid spike domain at 2.73 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-21
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	61.203, 86.315, 108.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.040 - 2.730
R-factor	0.2737
Rwork	0.269
R-free	0.31230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.597
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.040	2.860
High resolution limit [Å]	2.730	2.730
Number of reflections	15883	1541
<I/σ(I)>	7.6
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.989	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295.15	0.1 M HEPES pH 7.5, 27.5% PEG3350