8UDS

The Crystal Structure of CoxG from M. smegmatis, minus lipid anchoring C-terminus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-14
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	64.095, 64.095, 87.539
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.250 - 1.500
R-factor	0.1834
Rwork	0.181
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.320	1.520
High resolution limit [Å]	1.500	1.500
Rmerge	0.063	4.240
Rpim	0.013	0.075
Number of reflections	29933	1439
<I/σ(I)>	33.4
Completeness [%]	100.0
Redundancy	38.6
CC(1/2)	1.000	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.9	298	0.1 M KSCN (Salt) 30 %w/v PEG MME 2K (Precipitant)