8UD7

Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	210.030, 450.060, 623.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	182.490 - 2.550
R-factor	0.2073
Rwork	0.205
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhv
RMSD bond length	0.004
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	182.490	182.490	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.171	0.043	2.436
Rmeas	0.183	0.046	2.601
Total number of observations	14829135
Number of reflections	1891637	22074	139447
<I/σ(I)>	10.95	41.03	0.98
Completeness [%]	100.0	98.9	100
Redundancy	7.839	7.839	8.084
CC(1/2)	0.996	0.997	0.293

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME