8UCY
Sterile Alpha Motif (SAM) domain from Tric1, Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-11 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.639, 92.121, 93.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.170 - 1.480 |
R-factor | 0.1801 |
Rwork | 0.179 |
R-free | 0.19810 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.163 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.200 | 1.510 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.055 | 1.310 |
Rpim | 0.015 | 0.403 |
Number of reflections | 42224 | 2085 |
<I/σ(I)> | 28.1 | 1.8 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 14.3 | 11.1 |
CC(1/2) | 1.000 | 0.671 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 2.0 M ammonium sulphate, 0.1 M Tris, 12 mg/mL SAM protein |
2 | MICROBATCH | 8.5 | 293.15 | 1.0 M ammonium sulphate, 0.1 M Tris, 6.15 mg/mL SeMet SAM protein (0.5uL:0.5uL drop under paratone oil) |
2 | MICROBATCH | 8.5 | 293.15 | 1.0 M ammonium sulphate, 0.1 M Tris, 6.15 mg/mL SeMet SAM protein (0.5uL:0.5uL drop under paratone oil) |
2 | MICROBATCH | 8.5 | 293.15 | 1.0 M ammonium sulphate, 0.1 M Tris, 6.15 mg/mL SeMet SAM protein (0.5uL:0.5uL drop under paratone oil) |
2 | MICROBATCH | 8.5 | 293.15 | 1.0 M ammonium sulphate, 0.1 M Tris, 6.15 mg/mL SeMet SAM protein (0.5uL:0.5uL drop under paratone oil) |