8UAI
Crystal structure of hetero hexameric hazelnut allergen Cor a 9
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-12-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 149.168, 91.093, 74.854 |
| Unit cell angles | 90.00, 90.32, 90.00 |
Refinement procedure
| Resolution | 45.800 - 1.920 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.22120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.987 |
| Data reduction software | HKL-2000 (v722) |
| Data scaling software | HKL-2000 (v722) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmeas | 0.111 | 0.661 |
| Rpim | 0.063 | 0.381 |
| Number of reflections | 142012 | 7049 |
| <I/σ(I)> | 10 | 1.66 |
| Completeness [%] | 94.5 | 93.2 |
| Redundancy | 2.6 | 2.5 |
| CC(1/2) | 0.984 | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Protein drops: 5 mM Tris pH 8.0, 0.M M NaCl. Well solution: 0.2M NaCl |
