8UA5

Crystal Structure of infected cell protein 0 (ICP0) from herpes simplex virus 1 (A636-Q776)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	97.150, 97.150, 76.721
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.450 - 2.450
R-factor	0.1943
Rwork	0.192
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.991
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_4336: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.450	2.550
High resolution limit [Å]	2.450	2.450
Rmerge	0.113	0.926
Rmeas	0.124	1.012
Rpim	0.048	0.402
Total number of observations	89786	9814
Number of reflections	14026	1562
<I/σ(I)>	13.1	1.6
Completeness [%]	99.9
Redundancy	6.4	6.3
CC(1/2)	0.998	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	PACT G3: 20% (w/v) PEG 3350, 100 mM Bis-Tris Propane pH 7.5, 200 mM NaI, 80% crystallization solution and 20% (v/v) PEG 200.