8U9R

STRUCTURAL BASIS OF TRANSCRIPTION: RNA POLYMERASE II SUBSTRATE BINDING AND METAL COORDINATION USING A FREE-ELECTRON LASER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE MFX
Synchrotron site	SLAC LCLS
Beamline	MFX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-08
Detector	SLAC ePix10k 2M
Wavelength(s)	1.30505
Spacegroup name	C 2 2 21
Unit cell lengths	217.195, 387.440, 276.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.970 - 3.340
R-factor	0.2554
Rwork	0.255
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.593
Data reduction software	cctbx.xfel
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.680	3.360
High resolution limit [Å]	3.300	3.300
Rmerge	0.600	0.740
Number of reflections	166660	5833
<I/σ(I)>	3.93
Completeness [%]	95.8
Redundancy	10.25
CC(1/2)	0.914	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	10-15 % tacsimate 100mM Hepes pH 7.5