8U7G

Crystal structure of CIB_13 beta-galactosidase from Cuniculiplasma divulgatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-12-12
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	52.800, 102.188, 97.542
Unit cell angles	90.00, 104.69, 90.00

Refinement procedure

Resolution	29.630 - 2.220
R-factor	0.1811
Rwork	0.179
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.260
High resolution limit [Å]	2.220	2.220
Rmerge	0.061	0.436
Rpim	0.036	0.215
Number of reflections	49087	2010
<I/σ(I)>	16.89	1.93
Completeness [%]	98.0	81.8
Redundancy	3.6	2.7
CC(1/2)		0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	25% PEG 3350, 0.2 M KCl, 0.1 M Tris 0.1 pH 9