8U7B

Crystal structure of Apo form of Short Prokaryotic Argonaute TIR-APAZ (SPARTA) heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979338
Spacegroup name	P 65 2 2
Unit cell lengths	197.181, 197.181, 183.407
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.710 - 2.660
R-factor	0.1823
Rwork	0.179
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.608
Data reduction software	XDS (Jan 10, 2022 (BUILT 20220110))
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX ((1.20rc3_4406: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	170.760	2.990
High resolution limit [Å]	2.660	2.660
Rmerge	0.148	1.767
Rpim	0.042	0.554
Number of reflections	40914	2047
<I/σ(I)>	13.7	1.7
Completeness [%]	96.2
Redundancy	13.3
CC(1/2)	0.999	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293.15	0.1 M sodium acetate, pH 4.5, 0.8 M sodium phosphate monobasic, 1.2 M potassium phosphate dibasic