8U79

Crystal Structure of Substrate Binding Protein of Thermus thermophilus TAXI-TRAP protein with L-glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953732
Spacegroup name	P 21 21 21
Unit cell lengths	51.547, 67.913, 81.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.510 - 1.110
Rwork	0.120
R-free	0.13780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.030
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.552	1.130
High resolution limit [Å]	1.110	1.110
Rmerge	0.080	1.449
Rmeas	0.087	0.061
Rpim	0.033	0.650
Number of reflections	112678	58184
<I/σ(I)>	14.1
Completeness [%]	99.7
Redundancy	12.8	11.2
CC(1/2)	0.999	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	Ammonium citrate dibasic, PEG 3350