8U77

Crystal structure of Taf14 in complex with Yng1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2022-04-08
Detector	RDI CMOS_8M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	46.780, 64.610, 64.490
Unit cell angles	90.00, 101.53, 90.00

Refinement procedure

Resolution	45.840 - 1.930
R-factor	0.1852
Rwork	0.182
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.820
Data reduction software	iMOSFLM
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.840	2.000
High resolution limit [Å]	1.930	1.930
Rmerge	0.046	0.150
Rmeas	0.066	0.212
Rpim	0.046	0.150
Number of reflections	28287	2781
<I/σ(I)>	10.92	4.36
Completeness [%]	99.8	99.64
Redundancy	27.2	26.2
CC(1/2)	0.995	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	40% PEG 400, 0.1 M Tris pH 8.5, 0.2 M LiSO4