8U60
Structure of Mango II variant2 aptamer bound to T01-6A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-22 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.97741 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 36.374, 186.217, 104.339 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.510 - 3.300 |
R-factor | 0.2445 |
Rwork | 0.241 |
R-free | 0.29010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.488 |
Data reduction software | DIALS (3.8) |
Data scaling software | DIALS (3.8) |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.510 | 3.418 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 5691 | 552 |
<I/σ(I)> | 7.67 | |
Completeness [%] | 99.8 | |
Redundancy | 12.3 | |
CC(1/2) | 0.993 | 0.770 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | Sodium malonate, polyethylene glycol 3,350 |