8U3N
Structure of P450Blt from Micromonospora sp. MW-13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95365 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.078, 95.748, 105.217 |
| Unit cell angles | 90.00, 92.63, 90.00 |
Refinement procedure
| Resolution | 47.870 - 2.150 |
| R-factor | 0.1951 |
| Rwork | 0.194 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.084 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.870 | 2.227 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.061 | 0.355 |
| Number of reflections | 65730 | 6446 |
| <I/σ(I)> | 5.63 | 0.98 |
| Completeness [%] | 99.6 | |
| Redundancy | 2 | |
| CC(1/2) | 0.994 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.1M Bis-Tris pH 7, 22% PEG3350, 0.05 KSCN |
