8U38

Structure of a bacterial multi-ubiquitin domain protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 31 2 1
Unit cell lengths	136.024, 136.024, 105.807
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	117.800 - 3.040
R-factor	0.2376
Rwork	0.236
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	117.800	3.220
High resolution limit [Å]	3.040	3.040
Rmerge	0.204	3.416
Number of reflections	22162	3521
<I/σ(I)>	14	0.9
Completeness [%]	100.0
Redundancy	10
CC(1/2)	0.997	0.433

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	100 mM CAPS pH 10.5, 0.2 M Li2SO4, 1.2 M NaH2PO4, and 0.8 M K2HPO4